Cellsonics | Single Cell Sample Prep Re-engineered
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Home Hero Single Cell Sample Prep Re-engineered Single Cell Sample Prep
Re-engineered

Home Hero Single Cell Sample Prep Re-engineered

Cellsonics has re-engineered single cell sample preparation to capture the full potential of each and every tissue specimen. The innovative SimpleFlow™ tissue dissociation system enables researchers to capture important single cells in a user- and cell-friendly method.

With the SimpleFlow system

  • Maintain the native gene expression profile across tissue and cell types.
  • Recover the full spectrum of heterogeneous cell populations from solid tissue samples in 4-12 minutes of hands-free processing.
  • Dissociate samples in cold temperatures without the use of enzymes.
SimpleFlow Tissue Dissociation System

Cellsonics has re-engineered single cell sample preparation to capture the full potential of each and every tissue specimen. The innovative SimpleFlow™ tissue dissociation system enables researchers to capture important single cells in a user- and cell-friendly method.

With the SimpleFlow system

  • Maintain the native gene expression profile across tissue and cell types.
  • Recover the full spectrum of heterogeneous cell populations from solid tissue samples in 4-12 minutes of hands-free processing.
  • Dissociate samples in cold temperatures without the use of enzymes.
SimpleFlow Tissue Dissociation System

Single cells from solid tissues in minutes with SimpleFlow

Unlike other tissue dissociation methods, the SimpleFlow system features a hands-free workflow and requires no technical training.

SimpleFlow versus Enzymatic tissue dissociation workflow
SimpleFlow versus Enzymatic tissue dissociation workflow

BLU™ energy technology ensures cell-friendly dissociation across cell types

Sample preparation should not compromise biologically relevant data. The combination of the SimpleFlow automated sample handling cartridge and Bulk Lateral Ultrasonic™ (BLU) energy enables researchers to focus on data collection, not sample preparation.

The SimpleFlow system automatically and gently dissociates fresh solid tissue specimens at cold temperatures using BLU technology to generate suspensions of single cells without the need for enzymatic digestion. Simply place the tissue specimen in the sample cartridge and then recover your cell suspension for downstream cell sorting, and single-cell data analysis methods such as RNA-Seq, FACS, and mass spectrometry.

Our simplified solution sets new standards for obtaining single cells from solid tissue with unparalleled speed, quality, and sample integrity. In addition, the novel BLU energy technology is integrated into a fluidic circuit in a single-use cartridge that provides a complete, automated dissociation workflow solution not possible using enzymatic methods.

Better cell population recovery with SimpleFlow

FACS analysis - SimpleFlow dissociation versus Enzymatic dissociation

FACS analysis of dissociated mouse liver cells with the SimpleFlow tissue dissociation system vs. enzymatic dissociation methods. Cell recovery of specific cell-types including CD45+ leukocytes (green boxes), and hepatocyte progenitor cells (blue boxes) was observed to be higher with the SimpleFlow system than with enzymatically-dissociated cells.

Better cell population recovery with SimpleFlow

FACS analysis - SimpleFlow dissociation versus Enzymatic dissociation

FACS analysis of dissociated mouse liver cells with the SimpleFlow tissue dissociation system vs. enzymatic dissociation methods. Cell recovery of specific cell-types including CD45+ leukocytes (green boxes), and hepatocyte progenitor cells (blue boxes) was observed to be higher with the SimpleFlow system than with enzymatically-dissociated cells.

SimpleFlow’s unique ability to maintain native tissue gene expression profiles

Bulk RNA-Seq gene expression

Bulk RNA-Seq gene expression data generated from tissue dissociated with the SimpleFlow system. Gene expression in SimpleFlow dissociated cells (blue lines) is closely aligned to that of the native non-dissociated tissue, whereas enzymatically-dissociated cells (green line) show genes that are both over- and under-expressed compared to the control tissue. Data have been normalized to the response from intact control tissue.

Bulk RNA-Seq gene expression

Bulk RNA-Seq gene expression data generated from tissue dissociated with the SimpleFlow system. Gene expression in SimpleFlow dissociated cells (blue lines) is closely aligned to that of the native non-dissociated tissue, whereas enzymatically-dissociated cells (green line) show genes that are both over- and under-expressed compared to the control tissue. Data have been normalized to the response from intact control tissue.

Bulk RNA-Seq gene expression profiles of genes associated with cell stress. Data from porcine liver tissue dissociated with the SimpleFlow system compared to enzymatically dissociated cells and intact whole tissue. Genes associated with cell stress signals are upregulated in enzymatically dissociated cells compared to control. Data have been normalized to the response from intact control tissue.

Bulk RNA-Seq gene expression data
Bulk RNA-Seq gene expression data

Bulk RNA-Seq gene expression profiles of genes associated with cell stress. Data from porcine liver tissue dissociated with the SimpleFlow system compared to enzymatically dissociated cells and intact whole tissue. Genes associated with cell stress signals are upregulated in enzymatically dissociated cells compared to control. Data have been normalized to the response from intact control tissue.

Want more information?

Contact us to learn more about our SimpleFlow tissue dissociation system and set up a demo.